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2007•Science and Technology of Cereals,oils and FoodsRequires access

Determination of rutin in buckwheat leaves by HPLC

Geng Zhong

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Abstract

HPLC was used to determine rutin content rapidly.The optimum chromatograph parameters are as follow: column as Kromasil C18(10μm,4.6mm×250mm),mobile phase as methanol-water-acetic acid(40∶60∶1,v/v/v),flow rate of the mobile phase 1.0mL/min,detection wavelength 330nm.colume temp.20℃,and the linear range is around 50～500μg/mL.R2=0.9999.The proposed method shows a higher accuracy and precision.

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What this paper is about

HPLC was used to determine rutin content rapidly.The optimum chromatograph parameters are as follow: column as Kromasil C18(10μm,4.6mm×250mm),mobile phase as methanol-water-acetic acid(40∶60∶1,v/v/v),flow rate of the mobile phase 1.0mL/min,detection wavelength 330nm.colume temp.20℃,and the linear range is around 50～500μg/mL.R2=0.9999.The proposed method shows a higher accuracy and precision.

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Available abstract

HPLC was used to determine rutin content rapidly.The optimum chromatograph parameters are as follow: column as Kromasil C18(10μm,4.6mm×250mm),mobile phase as methanol-water-acetic acid(40∶60∶1,v/v/v),flow rate of the mobile phase 1.0mL/min,detection wavelength 330nm.colume temp.20℃,and the linear range is around 50～500μg/mL.R2=0.9999.The proposed method shows a higher accuracy and precision.

Key concepts: Rutin, Chromatography, High-performance liquid chromatography, Acetic acid, Linear range, Methanol, Chemistry, Linear relationship

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