Research on Tissue Culture and Rapid Propagation Technology of Stevia rebaudiana

[Objective] The aim was to study the tissue culture and rapid propagation technology of Stevia rebaudiana.[Method] The best explants sterilization method,optimal media of adventitious bud proliferation and seedling rooting were screened by using shoot tips of S.rebaudiana as explants.[Result] The best explants sterilization method was to sterilized with 0.1%HgCl2 for 5-8 min after being treated with 75% alcohol for 20 s;the optimal adventitious bud proliferation medium was MS+1.0 mg/L 6-BA+0.1 mg/L NAA and the adventitious bud proliferation rate was 80%;the optimal seedling rooting medium was 1/2MS+0.1 mg/L 6-BA+0.1 mg/L NAA+0.2 mg/L IBA.[Conclusion] This study provided a scientific basis for the industrialized and large-scale production of S.rebaudiana.