Tissue Culture Propagation of High Quality Stevia rebaudiana Bertoni Strain "1096"

The effects of different combinations of growth substances and explants on callus induction,bud differentiation and root formation were investigated in rapid growth and high steviol-glucosides content Stevia rebaudiana Bertoni Strain 1096,which was screened by improved DNS method.The results showed that stem apex seemed to be the best explant for callus induction and bud differentiation,followed by stem segment with axillary bud and the third was leaf.Both the stem segment without axillary bud and root of plantlets could be induced to form calli but not adventitious buds.The optimal combinations of 6-BA for adventitious bud induction on stem apex was 1.0mg/L,on stem segment with axillary bud was 1.5mg/L on MS medium;The best adventitious buds induction medium from leaves was MS medium with 1.0mg/L 6-BA and 1.0mg/L IAA.The proliferation culture medium for bud differentiation was MS medium with 0.5 mg/L 6-BA and 0.05 mg/L NAA.Rooting medium is 1/4MS+IBA 0.1 mg/L+NAA 0.1mg/L +active carbon l.0g/L,the rooting rate reached up to 100%,transplant survive was 95%.