Study on extraction process of raspberry polysaccharide with compound enzyme method

Single enzyme(pectinase,cellulase,papain) method was used for extracting polysaccharide,determined by pectinase,cellulase and papain compound to extract the raspberry fruit polysaccharide,the content of polysaccharide was measured by phenol-sulfuric acid method.Based on the ratio of compound enzyme test,the mathematical regression model was established about the dependent variable(extraction yield of polysaccharide) and independent variables(dosage of compound enzymes,pH,temperature and extraction time),and the optimum extraction condition was obtained by response surface methodology(RSM) as followed,composite enzyme mixture(pectinase,cellulase,papain)=2.5??1.7??2.1,the dosage of compound enzyme was 2.6%,pH 4,temperature 55 ℃,extraction time 2.6,the actual value was 4.12%.