Genetic diversity analysis of Agropyron germplasm resources by ISSR

The genetic diversity of 33 Agropyron materials from both at home and abroad was analyzed by ISSR(inter-simple sequence repeats) markers.Eleven primers were selected from 93 primers which had clear polymorphic bands.A total of 84 bands of which 59 were polymorphic were obtained from the 33 materials.On average,each primer amplification site was 7.64.The genetic similarity coefficient of varieties was between 0.083 and 0.706,and showed rich genetic diversity.UPGMA cluster analysis gave a genetic similarity coefficient of 0.52 for boundaries and the 33 materials were divided into four types,with basic clustering in accord with geographical origin and regional distribution.