Genetic Diversity in Germplasm Resources of Cotton from Different Area Based on ISSR Markers

The genetic diversity of 48 cotton accessions were analyzed by using ISSR markers.11 primers were screened out of 60 primers.Total of 92 bands were detected,77 of them were polymorphic.The percentage of polymorphic bands was 83.70%.UPGMA Cluster analysis based on ISSR data showed that the genetic similarity(GS)coefficient of 48 accessions ranged from 0.27 to 0.93.48 germplasm resources could be categorized into four major groups,wild cotton of Zhanjiang and Lianjiang,Guangdong province,showed great genetic difference from other materials,fell into one group,belong to primitive group.G.barbadense cotton were obvious genetic diffrent from G.hirsutum,also fell into one group,and high similarity among the rest cultivars from different provinces formed other groups.The study indicated that ISSR molecular marker is an efficient tool with high polymorphism and stability for analysis the genetic diversity and relationship of cotton germplasm resources.