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2006•Henan Journal of Preventive MedicineRequires access

Determination of soybean isoflavones in health foods by HPLC

DU Peng-chen

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Abstract

objective To establish a method for the determination of soybean isoflavones in health foods by HPLC.Methods After sonicated for 30 min and filtered through the 0.45 μm filter,the samples were separated on a C18 reversed-phase colum(200×4.6 mm 5 μm) using methanol +wateras the mobile phase,with gradient elution and detected by fluorescence detector. Ex=366nm,Em=417nm.Results The content was linear over the range 0.5 μg/mL-30 μg/mL.Y=13924Y-8625,r=0.9995.The recovery of the added sample De was 90.2%-108.4%,RSD was between 0.93%-5.2%.Conclusion With its accuracy,sensitivity,reproducibility as well as the simplicity of sample preparation,the method was feasible for the determination of soybean isoflavones in health foods.

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What this paper is about

objective To establish a method for the determination of soybean isoflavones in health foods by HPLC.Methods After sonicated for 30 min and filtered through the 0.45 μm filter,the samples were separated on a C18 reversed-phase colum(200×4.6 mm 5 μm) using methanol +wateras the mobile phase,with gradient elution and detected by fluorescence detector. Ex=366nm,Em=417nm.Results The content was linear over the range 0.5 μg/mL-30 μg/mL.Y=13924Y-8625,r=0.9995.The recovery of the added sample De was 90.2%-108.4%,RSD was between 0.93%-5.2%.Conclusion With its accuracy,sensitivity,reproducibility as well as the simplicity of sample preparation,the method was feasible for the determination of soybean isoflavones in health foods.

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Available abstract

objective To establish a method for the determination of soybean isoflavones in health foods by HPLC.Methods After sonicated for 30 min and filtered through the 0.45 μm filter,the samples were separated on a C18 reversed-phase colum(200×4.6 mm 5 μm) using methanol +wateras the mobile phase,with gradient elution and detected by fluorescence detector. Ex=366nm,Em=417nm.Results The content was linear over the range 0.5 μg/mL-30 μg/mL.Y=13924Y-8625,r=0.9995.The recovery of the added sample De was 90.2%-108.4%,RSD was between 0.93%-5.2%.Conclusion With its accuracy,sensitivity,reproducibility as well as the simplicity of sample preparation,the method was feasible for the determination of soybean isoflavones in health foods.

Key concepts: Chromatography, Isoflavones, High-performance liquid chromatography, Reproducibility, Chemistry, Gradient elution, Health food, Methanol

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