Cloning and Sequencing of Y -Interferon Gene of Bama mini pig

The porcine peripheral blood lymphocytes were cultured in vitro. After ConA was used to stimulate the lymphocytes for 15h, total RNA was isolated from the lymphocytes.the mini porcine IFN-Y gene was amplified by reverse transcription polymerase chain reaction (RT-PCR). PCR product was cloned into PMD18-T vector. Consequently, a DNA clone was obtained encoding Bama mini pig IFN-Y of 558 bp in length, which contained a complete open reading frame of 501 bp. Bama mini porcine IFN-Y was composed of 166 amino acids, which predicted molecular weight of 19 420. Bama mini porcine IFN-Y has an amino acid homology of 74.9% with human IFN-Y, 86.5% with bovine, 86.9% with goat,81% with horse, 58.7% with dog and 63.3% with rat. The homology of IFN-Y was 100% at nucleotide level between Bama mini pig and other pigs.