Optimization of enzymatic hydrolysis protein from hot pressed rapeseed meal using central composite design

Protein peptide has some of the biological activity and easily absorbed,applications of peptide in special food,pharmaceutical and biodegradable plastics have a potential prospect.This paper taking rapeseed protein from hot pressed rapeseed meal by ultrasonic wave as raw materials,and it is enzymatic hydrolysis using alkali protease.The single factor experiments and central composite design were adopted to study the effects of substrate concentration,enzymatic hydrolysis temperature,enzyme activity,pH value on degree of hydrolysis,the optimum process conditions of enzymatic hydrolysis were determined,then enzymatic hydrolysis regression model were established,substrate concentration 3%,enzymatic hydrolysis temperature 46 ℃,enzyme activity12000U/g,pH value 9.57,enzymatic hydrolysis time3 h,the degree of hydrolysis of rapeseed protein by this new process was 23.96%.It will lay the better foundation for further enzymatic hydrolysis,and it is economical feasible.