2006•Pharmaceutical Journal of Chinese People's Liberation ArmyRequires access

Determination of Paeoniflorin in Compound Radix Paeoniae Alba Capsules by HPLC

Hui Tang

Abstract

Aim To establish an HPLC method for determining paeoniflorin in compound Radix paeoniae alba capsules.Method The HPLC separation was performed on ZORBAX-C 18 (4.6mm×250mm,5μm) column with a mixture acetonitrile -0.1% phosphoric acid solution (15∶85) as the mobile phase,at a flow rate of 1.2ml/min.Result Paeoniflorin showed a good linear relationship in the range of 0.183～1.098μg.The regression equation was C=7.671×10 -5A-0.003 25,r=0.999 7,and the average recovery was 100.35% while RSD was 1.4%.Conclusion The method is convenient,repeatable,accurate,specific and can be used for determination of paeoniflorin in compound Radix paeoniae alba capsules.

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Key concepts: Paeoniflorin, Chromatography, Radix (gastropod), Phosphoric acid, High-performance liquid chromatography, Chemistry, Organic chemistry, Biology