Experimental study of Tsugeki about analgesic anti-inflammatory effect

Objective To observe analgesic anti-inflammatory effect of Tsugeki.Methods 30 mouse were randomly divided into five groups,control group,Rotundine group [60 mg/(kg·d)],Tsugeki high-,middle-and low dose group [375,250,125 mg/(kg·d)].Control group received intragastric administration of distilled water [20 mg/(kg·d)].Other groups received intragastric administration of corresponding medicine,once a day for six days.Frequency of stretching and latency period was observed by acetic acid-induced inflammation in mouse abdomen.In another experimental including same grouping,medication and dose,pain threshold of mouse were observed by mouse pain model induced by hot plate stimulation.30 mouse were randomly divided into five groups,control group,indometacin group [12.5 mg/(kg·d)],Tsugeki high-,middle-and low dose group [375,250,125 mg/(kg·d)].Control group received intragastric administration of distilled water [20 mg/(kg·d)].Other groups received intragastric administration of corresponding medicine,once a day for six days.Abdominal permeability in mouse was recorded in each group.In another experimental including same grouping,medication and dose,swelling degree of ear in mouse were observed by xylene-induced ear swelling.Results Frequencies of stretching in Rotundine group,Tsugeki high-,middle-and low dose group were decreased as compared with that in control group(P0.01,P0.05).Latency period in Rotundine group,Tsugeki high-,middle-and low dose group were prolonged as compared with that in control group(P0.01,P0.05).Frequencies of stretching in Rotundine group was not so many as that in Tsugeki high-,middle-and low dose group(P0.01).Latency period in Tsugeki high dose group were prolonged as compared with that in Tsugeki middle dose group(P0.05).Pain threshold of mouse in Rotundine group,Tsugeki high-,and middle dose group were obviously raised in comparison with that in control group(P0.01,P0.05).Pain threshold of mouse in Rotundine group was higher than that in Tsugeki high-,middle-and low dose group(P0.05).OD in indometacin group,Tsugeki high-,middle-and low dose group were significantly decreased as compared with that in control group(P0.01,P0.05).OD in indometacin group was decreased as compared with that in Tsugeki high-,middle-and low dose group(P0.05).Swelling degree of ear in mouse in indometacin group was significantly decreased as compared with that in control group(P0.05).Swelling degree of ear in mouse in Tsugeki high-,middle-and low dose group was decreased as compared with that in control group(P0.05).Conclusion Tsugeki has analgesic anti-inflammatory effect,375 mg/(kg·d)dose effect was the best.