2011•Lishizhen Medicine and Materia Medica ResearchRequires access
Effect of Breviscapinum on the Activity of CYP3A4 in Rats
Lou Jian-shi
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Abstract
Objective To study the effect of breviscapinum on the activity of CYP3A4 in rats with midazolam as probe. Methods The rats were randomly divided into three groups: control group,low-dose group and high-dose group.The rats in the low-dose group and high-dose group were treated with breviscapinum powder injection by caudal vein at the dose of 0.18 mg/100g and 0.54mg/100g once a day for 10 days respectively,the rats in the control group were injected with normal saline.On the 11th day,all rats in each group were injected midazolam,then venous blood samples were collected at a set of timepoints.The plasma concentration of midazolam was determined by HPLC. Results In low-dose group and high-dose group,AUC,MRT and t1/2 of Midazolam were significantly higher than those of control group(P0.05),and CLs was significantly lower than that of control group(P0.05).There was no significant difference between low-dose group and high-dose group(P0.05). Conclusion Breviscapinum can significantly inhibit the activity of CYP3A4 in rats.