Effect of pseudolaric acid A on inhibition proliferation of three cell lines

OBJECTIVE To study effect and mechanism of PAA on tumor ceU line MGC80-3, SMMC-7721 and K562. METHODS PAA in different concentrations was added into the medium in which the cells were cultured. Cytotoxicity rate was detected by MTT assay. The apoptosis was examined by Hoechst 33342/PI staining and DNA agarose gel electrophoresis. RESULTS The cell growth was remarkably inhibited by PAA.IC50 values of PAA for MGC80-3, SMMC-7721 and K562 cell line were 12.500,3.125 and 12.500 μmol·L-1 respectively. The apoptotic rate was respectively 83.50 % , 44.50 % and 63.20 % after 48 h of exposure to PAA. DNA ladder appeared on 1.5% agarose gel electrophoresis. CONCLUSION PAA efficiently induced growth inhibition and apoptosis in MGC80-3, SMMC-7721 and K562 cell lines.