Effect of Salivanol acid B on proliferation of K562 Human cell line in vitro

To study the effects and mechanism of Salivanol acid B on K562 cell line.Salivanol acid B of different concentrations were added into the medium in which the cells were cultured. The changes of cell morphology were observed by phase contrast microscope. Cellulotoxic activity of Salivanol acid B was defected by MTT. The number of apoptotic cells was quantitatively assessed by fluorescent light microscope.The growth of K562 cell line was remarkably inhibited by Salivanol acid B. The IC_(50) values of Salivanol acid B for K562 cells was 2.5×10~(-5) mol/L. The inhibited rate and the rate of apoptotic cells were related with the medicine concentration.[Conclusion]Salivanol acid B can effectively inhibit the multiplication of K562 cell line.