2006•Yumi kexueRequires access

Callus Initiation and Regeneration from Immture Embryos of Maize

Feng Yulan

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Abstract

Callus was induced from immature embryos of six maize inbred lines on N6 medium supplemented with various phytohormones.The results indicated that 2,4-dichlorophenoxyacetic acid(2,4-D) was necessary in inducing callus,and its concentration 2.0-2.5 mg/L.Different maize varieties had different regeneration capacities,the induction rates of Zong 3,Zheng 58 and K12 were over 85%;that of 137 and 87-1 ranged from50% to 60%;but that of the chang 7-2 was low 25%.

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Callus was induced from immature embryos of six maize inbred lines on N6 medium supplemented with various phytohormones.The results indicated that 2,4-dichlorophenoxyacetic acid(2,4-D) was necessary in inducing callus,and its concentration 2.0-2.5 mg/L.Different maize varieties had different regeneration capacities,the induction rates of Zong 3,Zheng 58 and K12 were over 85%;that of 137 and 87-1 ranged from50% to 60%;but that of the chang 7-2 was low 25%.

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Available abstract

Callus was induced from immature embryos of six maize inbred lines on N6 medium supplemented with various phytohormones.The results indicated that 2,4-dichlorophenoxyacetic acid(2,4-D) was necessary in inducing callus,and its concentration 2.0-2.5 mg/L.Different maize varieties had different regeneration capacities,the induction rates of Zong 3,Zheng 58 and K12 were over 85%;that of 137 and 87-1 ranged from50% to 60%;but that of the chang 7-2 was low 25%.

Key concepts: Callus, Regeneration (biology), Embryo, Biology, Inbred strain, 2,4-Dichlorophenoxyacetic acid, Tissue culture, Botany

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