Application of microwave technique to analysis with HPLC to Rupixiao tablets

Objective:To determine the content of notoginsenoside R1 and ginsenoside Rg1 in Rupixiao tablets by HPLC after applying microwave technique to deal with the sample.Methods:The distilled water as the solvent,to extract notoginsenoside R1 and ginsenoside Rg1 in Rupixiao tablets with the microwave circumfluence,and receiving the sample solution,an Agilent Eclipse XDB-C18 column(4.6 mm×150 mm,5 μm)was adopted with a mixture of acetonitrile and 0.05% phosphoric acid solution(20∶80)as the mobile phase at a flow rate of 0.72 mL·min-1 and the column temperature of 20 ℃ with the detection wavelength at 203 nm,the reference wavelength of 360 nm,and the whole wavelength scanning between 400 nm and 190 nm.Results:With this chromatographic condition,notoginsenoside R1 and ginsenoside Rg1 can be separated completely.The calibration curve was linear in the range of 49.25-492.5 μg·mL-1 for notoginsenoside R1 with r=0.9999 and 53.00-530.0 μg·mL-1 for ginsenoside Rg1 with r=0.9999;The average recoveries(n=6)of notoginsenoside R1 and ginsenoside Rg1 were 98.19% and 101.5%,respectively.Conclusion:The method is simple,accurate,and reproducible,the primary characteristic is fast,saving on the energy and the solvent,and the contamination is fewer,so it can reduce the labor intensity and improve the work circumstance.