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2015•Guangdong nongye kexueOpen access

Cloning and bioinformatics analysis of KAP1.1 gene in Tianzhu white yak

Zhang Liang-bi

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Abstract

Taking Keratin associated protein 1.1(KAP1.1) gene of yak as the research object,a specific primer wasdesigned according to the m RNA sequence of cattle KAP1.1 gene(Accession No.NM_001105369.1).Yak's complete CDS sequences of KAP1.1 were obtained by RT-PCR,PCR and cloning,the sequences were submitted to NCBI(Accession No.KJ095199) and analyzed by bioinformatics.The results showed that,the length of CDS sequences in yak KAP1.1 gene was 501 bp encoding 166 amino acids which belonged to hydrophilic protein.The secondary structures were mainly composed of the alpha helix,extended strand,Beta turn and random coil.The amino acid sequence had the highest homology with cattle,and it had the complete family proteinsdomain of High sulphur keratin-associated protein.

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What this paper is about

Taking Keratin associated protein 1.1(KAP1.1) gene of yak as the research object,a specific primer wasdesigned according to the m RNA sequence of cattle KAP1.1 gene(Accession No.NM_001105369.1).Yak's complete CDS sequences of KAP1.1 were obtained by RT-PCR,PCR and cloning,the sequences were submitted to NCBI(Accession No.KJ095199) and analyzed by bioinformatics.The results showed that,the length of CDS sequences in yak KAP1.1 gene was 501 bp encoding 166 amino acids which belonged to hydrophilic protein.The secondary structures were mainly composed of the alpha helix,extended strand,Beta turn and random coil.The amino acid sequence had the highest homology with cattle,and it had the complete family proteinsdomain of High sulphur keratin-associated protein.

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Available abstract

Taking Keratin associated protein 1.1(KAP1.1) gene of yak as the research object,a specific primer wasdesigned according to the m RNA sequence of cattle KAP1.1 gene(Accession No.NM_001105369.1).Yak's complete CDS sequences of KAP1.1 were obtained by RT-PCR,PCR and cloning,the sequences were submitted to NCBI(Accession No.KJ095199) and analyzed by bioinformatics.The results showed that,the length of CDS sequences in yak KAP1.1 gene was 501 bp encoding 166 amino acids which belonged to hydrophilic protein.The secondary structures were mainly composed of the alpha helix,extended strand,Beta turn and random coil.The amino acid sequence had the highest homology with cattle,and it had the complete family proteinsdomain of High sulphur keratin-associated protein.

Key concepts: Gene, Cloning (programming), Biology, YAK, Homology (biology), Genetics, Accession number (library science), Molecular biology

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Cloning and bioinformatics analysis of KAP1.1 gene in Tianzhu white yak — Research Paper | ScholarLens