Cloning and bioinformatics analysis on CDS of CAPN3 gene in yak

The CDS region and partial sequence of 3′UTR of the yak CAPN3 gene were cloned using RT-PCR with specific primers that designed according to cDNA sequence of bovine CAPN3 gene in the GenBank so as to analyze the bioinformatics of putative protein.The results showed that the yak CAPN3 gene contained a 2 469bp CDS region(GenBank accession no:JX112349.1)and encoded a putative 822 amino acids(GenBank accession no:AFP73395.1).Ten SNPs and corresponding three amino acid mutations in function region of protein were identified through alignment with bovine CAPN3 gene sequence.The putative molecular weight and theory isoelectric point of CAPN3 gene in yak were 94.58ku and 5.70,respectively.There was a hydrophobic region inⅢregion of yak CAPN3.The putative secondary structure of CAPN3 was mixed type with mainlyα-helices and random coil.Deduced amino acid sequences of CAPN3 gene between yak and bovine,sheep,wild boar and other species were high on homology and the phylogenetic distance consistent with their genetic relationship.