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2008•Progress in Veterinary MedicineRequires access

Cloning and Sequence Analysis of Yak Interleukin-4 gene

Zhizhong Jing

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Abstract

Cloning and genetic analysis of yak interleukin-4 gene were conducted.The full length cDNA of boIL-4 was amplified by RT-PCR from the yak peripheral blood lymphocyte stimulated by ConA and LPS,and was cloned in pMD18-T vector and sequenced.Homologies of nucleotide and deduced amino acid sequences between the yak IL-4 and registered boIL4 in the GenBank are 99.8% and 100%,respectively.Homologies of yak IL-4 nucleotide sequence compared with Homo,Macaca,Sus scrofa,Capra and Equus are in 44.4%-99.8%.Homologies of amino acid sequence of them are in 27.4%-91.1%.In conclusion,IL-4 containing an ORF of 408 bp and coding 135 amino acids was first cloned successfully from the yak PBMC in China.

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What this paper is about

Cloning and genetic analysis of yak interleukin-4 gene were conducted.The full length cDNA of boIL-4 was amplified by RT-PCR from the yak peripheral blood lymphocyte stimulated by ConA and LPS,and was cloned in pMD18-T vector and sequenced.Homologies of nucleotide and deduced amino acid sequences between the yak IL-4 and registered boIL4 in the GenBank are 99.8% and 100%,respectively.Homologies of yak IL-4 nucleotide sequence compared with Homo,Macaca,Sus scrofa,Capra and Equus are in 44.4%-99.8%.Homologies of amino acid sequence of them are in 27.4%-91.1%.In conclusion,IL-4 containing an ORF of 408 bp and coding 135 amino acids was first cloned successfully from the yak PBMC in China.

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Available abstract

Cloning and genetic analysis of yak interleukin-4 gene were conducted.The full length cDNA of boIL-4 was amplified by RT-PCR from the yak peripheral blood lymphocyte stimulated by ConA and LPS,and was cloned in pMD18-T vector and sequenced.Homologies of nucleotide and deduced amino acid sequences between the yak IL-4 and registered boIL4 in the GenBank are 99.8% and 100%,respectively.Homologies of yak IL-4 nucleotide sequence compared with Homo,Macaca,Sus scrofa,Capra and Equus are in 44.4%-99.8%.Homologies of amino acid sequence of them are in 27.4%-91.1%.In conclusion,IL-4 containing an ORF of 408 bp and coding 135 amino acids was first cloned successfully from the yak PBMC in China.

Key concepts: GenBank, Biology, Cloning (programming), Molecular biology, Complementary DNA, Gene, Coding region, Nucleic acid sequence

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