Analysis of the Drug Resistance and Genotypes of Metallo-β-lactamases-producing Enterobacter Cloacae

Objective To investigate the drug resistance and genotypes of metallo-β-lactamases-producing(MBL) Enterobacter cloacae.Methods Enterobacter cloacae identified by VITEK-2 system,antimicrobial sensitivity tests were performed by K-B method.MBL producing bacteria were screened by the double disk synergy test,and the MBL encoded genes were amplified by Polymeras(PCR).The genotypeswere confirmed by PCR products electrophoresis.Results The resistant rates of ceftazidime,Cefoxitin,imipenem,Meropenem,amikacin,cefepime,ciprofloxacin were 52.5%,52.5%,92.5%,7.5%,5.5%,65.0%,12.5%,85.0%,respectively.Among 40strains,3(7.5%) strains produced MBL.Two strains carried the genotype IMP among the 3 metallo-β-lactamases-producing strains.Conclusion Clinical antibiotics using should be depended on the antimicrobial susceptibility test results.Because of MBL-producing strains exist,the drug resistance of Enterobacter cloacae to imipenem carbapenem antibiotics is increasing.