2011•Chinese Journal of Hospital PharmacyRequires access

Determination of the content of kinsenoside in Anoectochilus roxburghii (Wall.) Lindl by HPLC

Yonghui Zhang

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Abstract

OBJECTIVE To determine the main content of kinsenoside in Anoectochilus roxburghii(Wall.) Lindl by HPLC method.METHODS C18 column,stationary phase(4.6 mm × 250 mm: 4 μm,8 nm,80 A),methanol-acetonitrile-water(5∶5∶90) as mobile phase;the detection wavelength was 215 nm.RESULTS The content of kinsenoside in wild Anoectochilus was 16.57%.Sampling in the concerntration range of 3.245-10.35 mg·mL-1,a good linear relationship was obtained, the regression equation Y=248.53X+30.37,r=0.999 3, average recovery was 97.26%,RSD was 1.74%.CONCLUSION The method is simple,rapid,reproducible,and sensitive that can be used for determination of the content of kinsenoside

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OBJECTIVE To determine the main content of kinsenoside in Anoectochilus roxburghii(Wall.) Lindl by HPLC method.METHODS C18 column,stationary phase(4.6 mm × 250 mm: 4 μm,8 nm,80 A),methanol-acetonitrile-water(5∶5∶90) as mobile phase;the detection wavelength was 215 nm.RESULTS The content of kinsenoside in wild Anoectochilus was 16.57%.Sampling in the concerntration range of 3.245-10.35 mg·mL-1,a good linear relationship was obtained, the regression equation Y=248.53X+30.37,r=0.999 3, average recovery was 97.26%,RSD was 1.74%.CONCLUSION The method is simple,rapid,reproducible,and sensitive that can be used for determination of the content of kinsenoside

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Available abstract

OBJECTIVE To determine the main content of kinsenoside in Anoectochilus roxburghii(Wall.) Lindl by HPLC method.METHODS C18 column,stationary phase(4.6 mm × 250 mm: 4 μm,8 nm,80 A),methanol-acetonitrile-water(5∶5∶90) as mobile phase;the detection wavelength was 215 nm.RESULTS The content of kinsenoside in wild Anoectochilus was 16.57%.Sampling in the concerntration range of 3.245-10.35 mg·mL-1,a good linear relationship was obtained, the regression equation Y=248.53X+30.37,r=0.999 3, average recovery was 97.26%,RSD was 1.74%.CONCLUSION The method is simple,rapid,reproducible,and sensitive that can be used for determination of the content of kinsenoside

Key concepts: Chromatography, Chemistry, Content determination, High-performance liquid chromatography, Acetonitrile, Content (measure theory), Methanol, Mathematics

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