Determination of the content of kinsenoside in Anoectochilus roxburghii (Wall.) Lindl by HPLC
Yonghui Zhang
Abstract
Yonghui Zhang
Abstract
OBJECTIVE To determine the main content of kinsenoside in Anoectochilus roxburghii(Wall.) Lindl by HPLC method.METHODS C18 column,stationary phase(4.6 mm × 250 mm: 4 μm,8 nm,80 A),methanol-acetonitrile-water(5∶5∶90) as mobile phase;the detection wavelength was 215 nm.RESULTS The content of kinsenoside in wild Anoectochilus was 16.57%.Sampling in the concerntration range of 3.245-10.35 mg·mL-1,a good linear relationship was obtained, the regression equation Y=248.53X+30.37,r=0.999 3, average recovery was 97.26%,RSD was 1.74%.CONCLUSION The method is simple,rapid,reproducible,and sensitive that can be used for determination of the content of kinsenoside
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OBJECTIVE To determine the main content of kinsenoside in Anoectochilus roxburghii(Wall.) Lindl by HPLC method.METHODS C18 column,stationary phase(4.6 mm × 250 mm: 4 μm,8 nm,80 A),methanol-acetonitrile-water(5∶5∶90) as mobile phase;the detection wavelength was 215 nm.RESULTS The content of kinsenoside in wild Anoectochilus was 16.57%.Sampling in the concerntration range of 3.245-10.35 mg·mL-1,a good linear relationship was obtained, the regression equation Y=248.53X+30.37,r=0.999 3, average recovery was 97.26%,RSD was 1.74%.CONCLUSION The method is simple,rapid,reproducible,and sensitive that can be used for determination of the content of kinsenoside
Key concepts: Chromatography, Chemistry, Content determination, High-performance liquid chromatography, Acetonitrile, Content (measure theory), Methanol, Mathematics