Determination of total flavanoid in tissue cultured anoectochilus roxburghii (wall.) lindl from Fujian by spectrofluorimetry

Objective To establish the Spectrofluorimetry of determining total flavanoid in tissue cultured Anoectochilus roxburghii(Wall.)Lindl.from Fujian.Methods On the basis of reaction of total flavanoid with alumimum ion to form fluorescent material,using 95% ethanol by ultrasonic wave to extract total flavanoid in tissue cultured Anoectochilus roxburghii(Wall.)Lindl from Fujian,a fluorimetric method for determination of total flavanoid was proposed by taking quecetin as standard sample.Results Under the optimum conditions,a linear relationship was obtained between the fluorescence intensity and the concentration of quercetin in the range of 0.002 ～ 0.400μg/mL,and the detection limit was 0.000 4μg/mL,linearity relationship was F=2002.6C+5.5296,correlativity was 0.9995,determination of total flavanoid in tissue cultured anoectochilus roxburghii(wall.)lindl from Fujian(according to the dry product calculation) was 73.98 μg/g,average rate of recovery was 99.95%,RSD was 0.92%(n=5).Conclusion This method is simple,rapid,accurate which is suitable for determining total flavanoid in tissue cultured Anoectochilus roxburghii(wall.)lindl.from Fujian.