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Determination of Total Flavanoid in WildAnoectochilus roxburghii (Wall.) Lindl.from Fujian by Spectrofluorometry

WU Shui-hua

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Abstract

Objective:The method of determining total flavanoid in wild Anoectochilus roxburghii (Wall.)Lindl.based on thereaction of flavanoid and alumimum(Ⅲ).Methods:The total flavanoid extracted from the samples with 95% ethanol under sonication.The content was measured by spectrofluorometry with quecetin as control.The recovery was also studied.Results:On the optimal conditions,the fluorescence intensity variations showed a linear relationshi Pwith the concentration of quercetin at 2~400 ng · mL-1.Detection limit was 0.4 ng · mL-1 (signal-to-noise ratio of 3,n=11).The linear regression equation was Y=2 098.1 X+4.167 8 (r=0.999 8),the recovery of 98.4% ( RSD=1.5%).Conclusion:The proposed method,which is suitable for the measurement of the total flavanoid in wild Anoectochilus roxburghii (Wall.)Lindl,is simple,rapid and reliable.

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What this paper is about

Objective:The method of determining total flavanoid in wild Anoectochilus roxburghii (Wall.)Lindl.based on thereaction of flavanoid and alumimum(Ⅲ).Methods:The total flavanoid extracted from the samples with 95% ethanol under sonication.The content was measured by spectrofluorometry with quecetin as control.The recovery was also studied.Results:On the optimal conditions,the fluorescence intensity variations showed a linear relationshi Pwith the concentration of quercetin at 2~400 ng · mL-1.Detection limit was 0.4 ng · mL-1 (signal-to-noise ratio of 3,n=11).The linear regression equation was Y=2 098.1 X+4.167 8 (r=0.999 8),the recovery of 98.4% ( RSD=1.5%).Conclusion:The proposed method,which is suitable for the measurement of the total flavanoid in wild Anoectochilus roxburghii (Wall.)Lindl,is simple,rapid and reliable.

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Available abstract

Objective:The method of determining total flavanoid in wild Anoectochilus roxburghii (Wall.)Lindl.based on thereaction of flavanoid and alumimum(Ⅲ).Methods:The total flavanoid extracted from the samples with 95% ethanol under sonication.The content was measured by spectrofluorometry with quecetin as control.The recovery was also studied.Results:On the optimal conditions,the fluorescence intensity variations showed a linear relationshi Pwith the concentration of quercetin at 2~400 ng · mL-1.Detection limit was 0.4 ng · mL-1 (signal-to-noise ratio of 3,n=11).The linear regression equation was Y=2 098.1 X+4.167 8 (r=0.999 8),the recovery of 98.4% ( RSD=1.5%).Conclusion:The proposed method,which is suitable for the measurement of the total flavanoid in wild Anoectochilus roxburghii (Wall.)Lindl,is simple,rapid and reliable.

Key concepts: Chemistry, Detection limit, Chromatography, Quercetin, Linear regression, Mathematics, Statistics, Biochemistry

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Determination of Total Flavanoid in WildAnoectochilus roxburghii (Wall.) Lindl.from Fujian by Spectrofluorometry — Research Paper | ScholarLens