The inhibitive effect and Bcl-2 expression of lymphoma cell line Raji with adriamycin and hyperthermia in vitro

Objective: To observe the inhibitive effect,apoptosis and Bcl-2 expression of Raji cells with adriamycin and hyperthermia. Method: The working concentration of adriamycin against Raji was determined by MTT assay that has been developed for quantitative evaluation of the proliferation of cells. Hyperthermia and chemotherapy were used singly or concurrently and the cell survival rates were obtained at 48h.The inhibitive effect was evaluated by MTT.The apoptosis rates and Bcl-2 expression of Raji were determined by flow cytometric analyses. Result: The concentration of adriamycin in the experiment was defined as its 48h IC50. The hyperthermia of 40℃,41℃ and 42℃ for 60min showed obvious inhibition to Raji cells(P0.01).Adriamycin chemotherapy combined with hyperthermia showed obvious inhibitive effect to Raji(P0.01). According to flow cytometric analyses,the treatment of hyperthermia and adriamycin used alone or concurrently could significantly increase the apoptosis rates and down-regulate expression of Bcl-2 of Raji cells(P0.01). Conclusion: Adriamycin chemotherapy combined with 60min of hyperthermia showed obvious inhibitive effect to Raji. This effect could increase the apoptosis rates and down-regulate expression of Bcl-2.