Method and its significance of confirming cut off values in ELISA

Objective To confirm the cut off value of commonly used laboratory reagents in ELISA.Methods According to kit introduction and related SOP,the varied verification projects were detected.The mean,standard deviation and x-±3sd were calculated and compared with those calculated by the kit provided method.If x-±3sd value of project closed to that provide by the kit,the cut off value provided by kit was suitable.Results For HBsAg,anti-HBsAg,anti-HBs,anti-HBe,anti-HBe,anti-HBc,anti-HBc-IgM,anti-HIV,anti-HCV-IgG,anti-TP,Pre-S1Ag,anti-HCV,the confirmation results were:0.057,0.125,0.078,0.730,0.765,0.032,0.070,0.037,0.097,0.047,0.017.Conclusion The confirmation results of anti-HBs,anti-HBe,anti-HBc close to those provided by kit and their cut off values are suitable,and the other cut off valuse are usnsuitable due to big discrepancy from the kit′s.