Determination of the cut-off limit for virus mark detecting in pool plasma samples
Jiang Yan-fan
Abstract
Jiang Yan-fan
Abstract
Assurance the result exact and reliable for pool plasma analysis for decrease the risk of virus infection. Using test kits from different vendor to determine of a cut-off limit for virus mark detecting in pool plasma samples. The double-antibody sandwich ELISA technique was used for HBsAg and anti-HIV-1 / HIV-2 test. The ELISA technique was used for anti-HCV test. The HBsAg cut-off limits for virus mark detecting of two vendor kits are 23. 398% and 26. 845%,The anti-HIV-1 / HIV-2 cut-off limits are 20. 025% and 23. 424%,The anti-HCV cut-off limits are 9. 012% and 16. 481%. Cut-off limits of two vendor's test kits were determined,and the limits can be used to judge detecting results of pool plasma samples.
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Assurance the result exact and reliable for pool plasma analysis for decrease the risk of virus infection. Using test kits from different vendor to determine of a cut-off limit for virus mark detecting in pool plasma samples. The double-antibody sandwich ELISA technique was used for HBsAg and anti-HIV-1 / HIV-2 test. The ELISA technique was used for anti-HCV test. The HBsAg cut-off limits for virus mark detecting of two vendor kits are 23. 398% and 26. 845%,The anti-HIV-1 / HIV-2 cut-off limits are 20. 025% and 23. 424%,The anti-HCV cut-off limits are 9. 012% and 16. 481%. Cut-off limits of two vendor's test kits were determined,and the limits can be used to judge detecting results of pool plasma samples.
Key concepts: Detection limit, Virology, Virus, HBsAg, Limit (mathematics), Cut-off, Human immunodeficiency virus (HIV), Chromatography