2014Chemical Research and ApplicationRequires access

Determination of the cut-off limit for virus mark detecting in pool plasma samples

Jiang Yan-fan

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Abstract

Assurance the result exact and reliable for pool plasma analysis for decrease the risk of virus infection. Using test kits from different vendor to determine of a cut-off limit for virus mark detecting in pool plasma samples. The double-antibody sandwich ELISA technique was used for HBsAg and anti-HIV-1 / HIV-2 test. The ELISA technique was used for anti-HCV test. The HBsAg cut-off limits for virus mark detecting of two vendor kits are 23. 398% and 26. 845%,The anti-HIV-1 / HIV-2 cut-off limits are 20. 025% and 23. 424%,The anti-HCV cut-off limits are 9. 012% and 16. 481%. Cut-off limits of two vendor's test kits were determined,and the limits can be used to judge detecting results of pool plasma samples.

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What this paper is about

Assurance the result exact and reliable for pool plasma analysis for decrease the risk of virus infection. Using test kits from different vendor to determine of a cut-off limit for virus mark detecting in pool plasma samples. The double-antibody sandwich ELISA technique was used for HBsAg and anti-HIV-1 / HIV-2 test. The ELISA technique was used for anti-HCV test. The HBsAg cut-off limits for virus mark detecting of two vendor kits are 23. 398% and 26. 845%,The anti-HIV-1 / HIV-2 cut-off limits are 20. 025% and 23. 424%,The anti-HCV cut-off limits are 9. 012% and 16. 481%. Cut-off limits of two vendor's test kits were determined,and the limits can be used to judge detecting results of pool plasma samples.

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Available abstract

Assurance the result exact and reliable for pool plasma analysis for decrease the risk of virus infection. Using test kits from different vendor to determine of a cut-off limit for virus mark detecting in pool plasma samples. The double-antibody sandwich ELISA technique was used for HBsAg and anti-HIV-1 / HIV-2 test. The ELISA technique was used for anti-HCV test. The HBsAg cut-off limits for virus mark detecting of two vendor kits are 23. 398% and 26. 845%,The anti-HIV-1 / HIV-2 cut-off limits are 20. 025% and 23. 424%,The anti-HCV cut-off limits are 9. 012% and 16. 481%. Cut-off limits of two vendor's test kits were determined,and the limits can be used to judge detecting results of pool plasma samples.

Key concepts: Detection limit, Virology, Virus, HBsAg, Limit (mathematics), Cut-off, Human immunodeficiency virus (HIV), Chromatography

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