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Method and its significance of confirming cut-off values in ELISA test before blood transfusion

Geng Xiu-rong

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Abstract

Objective To confirm the cut off value of commonly used laboratory reagents in ELlSA.Methods Samples are detected according to Their kit introduction and related SOP.The mean,standard deviation and X±3sd were calculated and compared with those calculated by the kit provided.If X±3sd value of project closed to that provide by the kit,the cut off value provided by kit was suitable.Results For HBsAg,anti-HIV,anti-HCV-IgG,anti-TP,the confirmation results were:0.0554、0.0552、0.1753、0.1382,They are different from the values provided by the kit.Conclusion The cut-off valuses of HBsAg、Anti-HIV、Anti-HCV-IgG and anti-TP are usnsuitable due to big discrepancy from the kit.

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What this paper is about

Objective To confirm the cut off value of commonly used laboratory reagents in ELlSA.Methods Samples are detected according to Their kit introduction and related SOP.The mean,standard deviation and X±3sd were calculated and compared with those calculated by the kit provided.If X±3sd value of project closed to that provide by the kit,the cut off value provided by kit was suitable.Results For HBsAg,anti-HIV,anti-HCV-IgG,anti-TP,the confirmation results were:0.0554、0.0552、0.1753、0.1382,They are different from the values provided by the kit.Conclusion The cut-off valuses of HBsAg、Anti-HIV、Anti-HCV-IgG and anti-TP are usnsuitable due to big discrepancy from the kit.

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Available abstract

Objective To confirm the cut off value of commonly used laboratory reagents in ELlSA.Methods Samples are detected according to Their kit introduction and related SOP.The mean,standard deviation and X±3sd were calculated and compared with those calculated by the kit provided.If X±3sd value of project closed to that provide by the kit,the cut off value provided by kit was suitable.Results For HBsAg,anti-HIV,anti-HCV-IgG,anti-TP,the confirmation results were:0.0554、0.0552、0.1753、0.1382,They are different from the values provided by the kit.Conclusion The cut-off valuses of HBsAg、Anti-HIV、Anti-HCV-IgG and anti-TP are usnsuitable due to big discrepancy from the kit.

Key concepts: Medicine, HBsAg, Human immunodeficiency virus (HIV), Value (mathematics), Virology, Immunology, Statistics, Virus

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Method and its significance of confirming cut-off values in ELISA test before blood transfusion — Research Paper | ScholarLens