Preparation and Application of Internal Quality Control Substance by Real-time PCR to Quantify Hepatitis B Virus DNA and Quality Control Chart

Objective To prepare an internal quality control substance of HBV-DNA used in real time quantitative PCR, and establish a statistics internal quality control system and plot quality control chart with Microsoft Office Excel.Methods HBV-DNA positive quality control serum was prepared and was subpackage several centrifuge tubes with 0.5ml,and saved subzero 70 degree.After detected 20 runs repeatly,mean value,standard deviation (s) and coefficient of variability (CV) were calculated and quality control chart was plotted.Results The log of mean value of HBV-DNA internal quality control is 5.573,standard deviation is 0.244,coefficient of variability is 4.4%.It is fit for dynamic state monitoring through be plotted warning line and out of control line with Excel.Conclusion Internal quality control substance of HBV DNA used in real-time quantitative PCR is very stable and applicable in clinical laboratory.