Effect of induced concentration of IPTG on the expression of recombinant human augmenter ofliver regeneration gene

The object is recombinant PET-A viz.recombinant of human augmenter of liver regeneration(hALR) gene with fusion expression vector pET28a(+) contained T7 promoter, transformed into E. coli BL21(DE3), induced and expressed by IPTG, analyzed the effect of different concentration of IPTG on expression product, confirmed the best induced concentration of IPTG. Experimental results indicated that hALR expression quantity was the most when ultima concentration of IPTG was 1.0 mmol·L-1. This provided dependable experimental accordance for industry production of recombinant gene engineering medicine when IPTG was inducer.