2004•Xibei zhiwu xuebaoRequires access
Establishment of efficient regeneration system from leaf explants of potato
Li Juan, Cheng Zhihui, Guoyu Zhang
Open publisher page 1 citations
Abstract
The regeneration system of four cultivated potatoes in vitro was studied with leaves as explants,and the results showed that the optimal medium for callus induction from leaf discs was MS+6-BA 2.5 mg·L~(-1)+NAA 0.2 mg·L~(-1) for “Dongnong 303” and “E No.1”,MS+6-BA 2.0 mg·L~(-1)+NAA 0.1 mg·L~(-1) for “Favorite” and MS+6-BA 2.0 mg·L~(-1)+NAA 0.2 mg·L~(-1) for “Sharpdi”.The optimal medium for adventitious bud differentiation from leaf-derived calluses was found to be MS+6-BA 2.5 mg·L~(-1)+GA_3 5.0 mg·L~(-1) for “Favorite” and “E No.1,MS+6-BA 1.0 mg·L~(-1)+IAA 0.1 mg·L~(-1)+GA_3 2.5 mg·L~(-1) for “Dongnong 303”,MS+6-BA 2.5 mg·L~(-1)+IAA 0.5 mg·L~(-1)+GA_3 2.5 mg·L~(-1) for “Sharpdi”.Adventitious bud differentiation rate of “Dongnong 303”,“Sharpdi”,“Favorite” and “E No.1” reached 94.3%,100%,100% and 90% respectively.