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Establishment of Efficient Regeneration System from Leaf Explants of Potato

Sui QiJun

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Abstract

The regeneration system of eight potato cultivars was studied with leafs as explants.The results showed that the optical callus induction medium was MS+6-BA 3 mg/L+NAA 0.3 mg/L+GA3 10 mg/L for Yunshu 501 and MS+6-BA 3 mg/L +NAA 0.5 mg/L +GA3 10 mg/L for Hui-2,and the induction rate reached to 100 % and 95.7 %,respectively.The optical shoot induction medium was found to be MS+6-BA 3mg/L +GA310 mg/L for Yunshu501 and MS+6-BA 3 mg/L+NAA 0.02 mg/L +GA3 10 mg/L for Hui-2,and the regeneration frequency reached to 86.3 % and 65.4 %,respectively.In contrast,no or very low callus regeneration was obtained from other cultivars,indicating that regeneration efficiency was strongly genotype-depended.

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What this paper is about

The regeneration system of eight potato cultivars was studied with leafs as explants.The results showed that the optical callus induction medium was MS+6-BA 3 mg/L+NAA 0.3 mg/L+GA3 10 mg/L for Yunshu 501 and MS+6-BA 3 mg/L +NAA 0.5 mg/L +GA3 10 mg/L for Hui-2,and the induction rate reached to 100 % and 95.7 %,respectively.The optical shoot induction medium was found to be MS+6-BA 3mg/L +GA310 mg/L for Yunshu501 and MS+6-BA 3 mg/L+NAA 0.02 mg/L +GA3 10 mg/L for Hui-2,and the regeneration frequency reached to 86.3 % and 65.4 %,respectively.In contrast,no or very low callus regeneration was obtained from other cultivars,indicating that regeneration efficiency was strongly genotype-depended.

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Available abstract

The regeneration system of eight potato cultivars was studied with leafs as explants.The results showed that the optical callus induction medium was MS+6-BA 3 mg/L+NAA 0.3 mg/L+GA3 10 mg/L for Yunshu 501 and MS+6-BA 3 mg/L +NAA 0.5 mg/L +GA3 10 mg/L for Hui-2,and the induction rate reached to 100 % and 95.7 %,respectively.The optical shoot induction medium was found to be MS+6-BA 3mg/L +GA310 mg/L for Yunshu501 and MS+6-BA 3 mg/L+NAA 0.02 mg/L +GA3 10 mg/L for Hui-2,and the regeneration frequency reached to 86.3 % and 65.4 %,respectively.In contrast,no or very low callus regeneration was obtained from other cultivars,indicating that regeneration efficiency was strongly genotype-depended.

Key concepts: Explant culture, Callus, Cultivar, Regeneration (biology), Shoot, Horticulture, Chemistry, Murashige and Skoog medium

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