DIFFERENCE IN NUCLEAR DNA BETWEEN CYTOPLASMIC MALE STERILE LINE A_2V_4 AND ITS MAINTAINER V_4 OF SORGHUM

The total DNA from cytoplasmic male sterile line A 2V 4 (A) and its maintainer V 4 (B) in sorghum were screened primarily by RAPD using 184 random sequence 10 mer primers, and from which 6 primers, amplifying reproducibly polymorphic bands between A/B at each time, were identified. Then the 6 primers were used further to amplify their total DNA, mitochondrial DNA (mtDNA) and chloroplast DNA (cpDNA), respectively. And 12, 4 and 11 polymorphic fragments, which were different between A and B, were produced when total DNA, mtDNA and cpDNA were used as template,respectively. Excepting the 7 fragments appeared only in the of mtDNA and cpDNA, 5 among the 12 fragments occurred in total DNA also amplified in the amplification of mtDNA and/or cpDNA. And these 12 fragments were thought amplified from cytoplasmic DNA because of the total DNA including nuclear DNA, mtDNA and cpDNA. In contrast, another 7 fragments, which appeared only in the amplification of total DNA and never in that of cytoplasmic DNA, were thought amplified from nuclear DNA. Among these 7 fragments, there are 5 occurred in maintainer V 4 and 2 in CMS line A 2V 4, respectively. These results indicate it is different in nuclear DNA between CMS line and its maintainer. The importance of nuclear DNA on CMS and the significance of this researching strategy were discussed. Fig 2, Tab 4, Ref 19