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2003•Chinese Journal of Appplied Environmental BiologyRequires access

CLONING ANG SEQUENCE ANALYSIS OF ps1A1 AND ps1A2 GENES AMPLIFIED SPECIFICALLY FROM THE CHLOROPLAST DNA OF MAINTAINER OF CMS SORGHUM

Yi Sun

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Abstract

The total DNAs from 15 allocytoplasmic or allonuclear sorghum species were analyzed by RAPD analysis, and a fragment SAY-19 2300 was amplified specifically from total DNAs of seven sorghum cultivars including 4 maintainers and 3 restorers with fertile (N) cytoplasm. PCR assays showed that this fragment was amplified from chloroplast (cp) DNA. The total DNA, mitochondrial (mt) DNA, and cpDNA were digested with EcoRI+HindIII, and hybridized with fragment SAY-19 2300 as probe. The hybridization signals appeared both in total DNA and cpDNA but not in mtDNA. No obviously and regularly polymorphic hybridization signals between the N- cytoplasm and sterile (S) cytoplasm were observed. Sequence analysis revealed this newly cloned fragment contained a portion of ps1A1 (1-2139bp) and ps1A2 (2165-2447bp) genes. Fig 6, Tab 2, Ref 19

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The total DNAs from 15 allocytoplasmic or allonuclear sorghum species were analyzed by RAPD analysis, and a fragment SAY-19 2300 was amplified specifically from total DNAs of seven sorghum cultivars including 4 maintainers and 3 restorers with fertile (N) cytoplasm. PCR assays showed that this fragment was amplified from chloroplast (cp) DNA. The total DNA, mitochondrial (mt) DNA, and cpDNA were digested with EcoRI+HindIII, and hybridized with fragment SAY-19 2300 as probe. The hybridization signals appeared both in total DNA and cpDNA but not in mtDNA. No obviously and regularly polymorphic hybridization signals between the N- cytoplasm and sterile (S) cytoplasm were observed. Sequence analysis revealed this newly cloned fragment contained a portion of ps1A1 (1-2139bp) and ps1A2 (2165-2447bp) genes. Fig 6, Tab 2, Ref 19

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Available abstract

The total DNAs from 15 allocytoplasmic or allonuclear sorghum species were analyzed by RAPD analysis, and a fragment SAY-19 2300 was amplified specifically from total DNAs of seven sorghum cultivars including 4 maintainers and 3 restorers with fertile (N) cytoplasm. PCR assays showed that this fragment was amplified from chloroplast (cp) DNA. The total DNA, mitochondrial (mt) DNA, and cpDNA were digested with EcoRI+HindIII, and hybridized with fragment SAY-19 2300 as probe. The hybridization signals appeared both in total DNA and cpDNA but not in mtDNA. No obviously and regularly polymorphic hybridization signals between the N- cytoplasm and sterile (S) cytoplasm were observed. Sequence analysis revealed this newly cloned fragment contained a portion of ps1A1 (1-2139bp) and ps1A2 (2165-2447bp) genes. Fig 6, Tab 2, Ref 19

Key concepts: Chloroplast DNA, EcoRI, Biology, Mitochondrial DNA, RAPD, Molecular biology, Cloning (programming), DNA

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CLONING ANG SEQUENCE ANALYSIS OF ps1A1 AND ps1A2 GENES AMPLIFIED SPECIFICALLY FROM THE CHLOROPLAST DNA OF MAINTAINER OF CMS SORGHUM — Research Paper | ScholarLens