Analysis of Differences between Nuclear DNA and Cytoplasmic DNA in Male Sterile Line and Fertile Line of Eggplant

The total DNA from male sterile line(MS) Zhongxin and its fertile line (CK) in eggplant were screened for 60 random sequences. And primers which amplified polymorphic bands reproducibly between MS and CK were indentified. Then the 5 primers were used to amplify their total DNA , nuclear DNA and mitochiondrial DNA(mtDNA). And 9, 5 and 9 polymorphic fragments ,which are different from S and F, were produced when total DNA, nuclear DNA and mtDNA were used as the template. 5 among the 9 fragments occurring in total DNA also amplified from mtDNA, but not from nuclear DNA, so the 5 fragments were thought to be amplified from mtDNA. Among these 5 fragments, there were 4 occurring in male sterile line and 1 in fertile line. These results indicated there were mtDNA differences between MS line and fertile line. 4 among the 9 fragments occurring in total DNA were also amplified from nuclear DNA, but not from mtDNA, so the 4 fragments were thought to be amplified from nuclear DNA. Among these 4 fragments, there were 3 occurring in male sterile line and 1 in fertile line. These results indicated there were nuclear DNA differences between MS and CK, and the male sterility of eggplant probably resulted from the interaction between cytoplasmic gene and nuclear gene.