Comparison of Different Methods for the Detection of Polydatin in RHIZOMA ET RADIX POLYGONI CUSPIDATI

[Objective] To compare the content of polydatin in artificial cultivated RHIZOMA ET RADIX POLYGONI CUSPIDATI determined by different methods.[Method] High-performance liquid chromatography(HPLC) and capillary electrophoresis(CE) were adopted to determine the content of polydatin in the artificial cultivars of RHIZOMA ET RADIX POLYGONI CUSPIDATI.Then,the linear range,quantitative reproducibility,detection limit and analysis time of the separation between the two methods were comparatively studied.[Result] High-performance liquid chromatography with fluorescence detection(HPLC-FD) was the optimum method for detecting polydatin in RHIZOMA ET RADIX POLYGONI CUSPIDATI.Following the injection of 10 μl of sample solution,polydatin was separated on a chromatographic column Diamonsil-C18(250 mm×0.46 mm,5 μm) with 25% acetonitrile as mobile phase at the column temperature of 25 ℃,and then quantified by fluorescence detection with the excitation wavelength at 334 nm and the emission wavelength at 408 nm.The flow rate of mobile phase was set to 1.0 ml/min.[Conclusion] HPLC is an ideal method to determine the content of polydatin in RHIZOMA ET RADIX POLYGONI CUSPIDATI.