Simultaneous Determination of Multiple Typical Ingredients in RHIZOMA POLYGONI CUSPIDATI

[Objective] To set up an analysis method for simultaneously determining the content of multiple typical ingredients in RHIZOMA POLYGONI CUSPIDATI so as to evaluate the quality of related medicinal materials produced in different areas.[Method] The HPLC system was established with reverse-phase C18 Hyperrsil column(250 mm×4.6 mm,5 μm),which was packed with the octadecyl silane chemically bonded silica.The mobile phase consisted of acetonitrile and 1%phosphoric acid water solution.Following the injection of test sample,a gradient elution was initiated at a flow rate of 1.0 ml/min: the ratio of acetonitrile in the mobile phase was from 20% to 38% at 0-30 min,and increased to 38% to 100% at 30-70 min.And the UV detective wavelength was set at 284 nm.[Result] Through investigation,polydatin,resverotol,emodin-8-O-β-D-glucopyranosid,emodin,chrysophanol and quistin displayed good linear relations within a certain range.The recovery rate ranged from 95.00% to 105.0%(n=5).[Conclusion] The method is simple and accurate,and can simultaneously determine the content of the stilbenes and anthraquinones in RHIZOMA POLYGONI CUSPIDATI,as well as providing a simple,feasible and typical analyzing method for the evaluation on the quality of Chinese medicinal materials RHIZOMA POLYGONI CUSPIDATI.