Comparison of the Content of Scutellarin in Erigeron breviscapus (Vant.) Hand Mazz. from Different Places of Yunnan Province
Yundong Shi
Abstract
Yundong Shi
Abstract
[Objective] To determine the content of scutellarin in Erigeron breviscapus(Vant.) Hand Mazz.from different places of Yunnan Province.[Method] The content of scutellarin was deter mined by HPLC,and the HPLC conditions were proposed as follows: column of Shim-pack VP-ODS(150.0 mm×4.6 mm,5 μm),mobile phase of CH3OH-0.1% phosphoric acid(40∶60,V/V),flow rate of 1.0 ml/ min,column temperature of 25 ℃and detection wavelength of 335 nm.[Result] Erigeron breviscapus(Vant.) Hand Mazz.from different places of Yunnan Province differed from each other in scutellarin,and those from Yaoshan,Zhaotong and Shuanglong Town of Kun ming were richest(2.628%) and lowest(1.597%) in scutellarin,respectively.[Conclusion]Good in precision and repeatability,the method established in this case is in favor of the deter mination of scutellarin in Erigeron breviscapus(Vant.) Hand Mazz.,and this study provides basis for the breeding of improved variety and GAP cultivation of Erigeron breviscapus(Vant.) Hand Mazz..
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[Objective] To determine the content of scutellarin in Erigeron breviscapus(Vant.) Hand Mazz.from different places of Yunnan Province.[Method] The content of scutellarin was deter mined by HPLC,and the HPLC conditions were proposed as follows: column of Shim-pack VP-ODS(150.0 mm×4.6 mm,5 μm),mobile phase of CH3OH-0.1% phosphoric acid(40∶60,V/V),flow rate of 1.0 ml/ min,column temperature of 25 ℃and detection wavelength of 335 nm.[Result] Erigeron breviscapus(Vant.) Hand Mazz.from different places of Yunnan Province differed from each other in scutellarin,and those from Yaoshan,Zhaotong and Shuanglong Town of Kun ming were richest(2.628%) and lowest(1.597%) in scutellarin,respectively.[Conclusion]Good in precision and repeatability,the method established in this case is in favor of the deter mination of scutellarin in Erigeron breviscapus(Vant.) Hand Mazz.,and this study provides basis for the breeding of improved variety and GAP cultivation of Erigeron breviscapus(Vant.) Hand Mazz..
Key concepts: Scutellarin, Chemistry, Mathematics, Chromatography