Protective effect of GLP on lung injury induced by hemorrhagic shock-reperfusion in rabbits

Objective To study the protective effect of ganoderma lucidum polysaccharide(GLP)on lung injury induced by hemorrhagic shock-reperfusion in rabbits.Methods The rabbits were divided into group S(sham operation),group NS(reperfusion with normal saline for 90 min after hemorrhagic shock),and group LS(reperfusion with 1% GLP instead of NS as in group NS).The rabbits were killed quickly at the end of reperfusion for measuring NO content and activity of NO synthase(NOS)and pathological examination.Results The activity of NOS and the content of NO in the lungs were lower in groups of NS and LS than those in group S,which were higher in group LS than those in group NS(P0.05).Leucocyte count in bronchoalveolar lavage fluid was more in group NS than that in group S and group LS(P0.05).The pathological results showed serious pulmonary structural injury occurred in group NS,but no significant injury was found in group LS.Conclusion GLP has a protective effect on lung reperfusion injury induced by HS-R through enhancing NOS activity and increasing NO production.