Effects of GLP on Intestinal Mucosa Injury and Changes of Nitric Oxide Content in Hemorrhagic Shock Rabbits

Objective To observe the intestinal mucosa injury and changes of nitric oxide(NO)content in hemorrhagic shock/reperfusion(HS-R)rabbits and the effect of ganoderma lucidum polysaccharide(GLP)on them.Methods With the model of HS-R in rabbits,the animals were divided into three groups:sham operation group(group S),reperfusion with NS group(group NS),reperfusion with 1% GLP group(group LS).The incidence rates of bacterial translocation were observed at the time of pre-shock,shock 40 min,post-reperfusion 40 min and 90 min respectively.NO content in serum and intestinal mucosa,the activity of NOS in intestinal mucosa,the degree of intestinal injury were examined at the time of post-resuscitation 90 min.Results ① With the extension of reperfusion time the positive rate of blood bacteria increased gradually in NS group,which was significantly higher than that of group S and group LS(P0.05),meanwhile the degree of intestinal injury in group NS was more severe than that of group S and group LS(P0.05).② NO content in serum of group NS and group LS was increased significantly compared with that before shock and in group S(P0.05).NO content in serum was further increased after reperfusion with NS,which was significantly higher than that in group LS.NO content and NOS activity in intestinal mucosa of group NS were significantly higher than those in group LS and group S(P0.05).Conclusion The intestinal mucosa injury in HS-R is related to the activation of NOS and the increase of No content,meanwhile GLP can reduce the intestinal mucosa injury in HS.