2005•Biotechnology(Faisalabad)Requires access
Callus Induction and Differentiation of Dioscorea panthaica
Guotao Yang
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Abstract
The Dioscorea panthaica callus induction and differentiation were studied with different hormones when it used the wild Dioscorea panthaica as explant. The result showed: Different explants would bring great difference of the induction rate, the induction rate of the blade was the highest, up to 85 .7%, the induction rate of the stem was relatively low, only about 10% equally. When it used blade as explant, the best callus induction formula was 2.0ml/L 2,4-D and 2.5ml/L 6-BA were added in the MS culture medium, the best bud differentiation formula was 0.5ml/L NAA and 1.0ml/L BA were added in the MS medium, with 2% sugar at pH6.4. And the root differentiation rate was highest when 0.1ml/L NAA and 1.0ml/L BA were added in it,with 3% sugar at pH6.8.