Determination of tylosin residues in fishery products by high performance liquid chromatography

Objective:To establish a HPLC method for determination of tylosin residue in fishery products.Methods: The samples were extracted by ethyl acetate at pH 8.0 medium,after condensed with rotary evaporation,the sample was extracted for defatting by hexane,and purified with SCX solid-phase extraction column.The target compound was separated with gradient mobile phase consisting of acetonitrile-phosphate buffer solution,and quantified by external standard method.Results: The method showed a good linearity within the range of 0.050 μg/ml~5.00 μg/ml with a correlation coefficient of 0.9998.The detection limit was 30 μg/kg and the quantification limits were 100 μg/kg.The mean recoveries at spiked concentration of 50 μg/kg~500 μg/kg were in the range of 72.4%~106%,and the relative standard deviations were less than 10%.Conclusion: The sensitivity and repeatability of the method could meet the analysis requirements for determination of tylosin residues in fishery products.