2006•Lishizhen Medicine and Materia Medica ResearchRequires access
Analysis of the Components in Callus and Cell Suspension Cultures of Ligusticum chuanxiongHort. by Gas Chromatography-Mass Spec-trometry
Jungui Dai
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Abstract
ObjectiveTo analyze the components in callus and cell suspension cultures ofLigusticum chuanx-iongHort.by gas chromatography-mass spectrometry(GC-MS).MethodsInitiate the callus and cell sus-pension cultures ofLigusticum chuanxiongHort..Collect the callus and three sequent generations cell suspen-sion cultures,and the components in callus and cell suspension cultures were analyzed by gas chromatography-mass spectrometry.ResultsThe callus and cell suspension cultures ofLigusticum chuanxiongHort.containedsimilar constituenents,but the components in callus and cell suspension cultures were quite different from thoseof the plant,and the main bioactive constituentes of the plant were not found in callus and cell suspension cul-tures.ConclusionThe characteristics of callus and cell suspension cultures were different from those of theplant.