Determination of Carbendazim Residue in Watermelon and Soil by HPLC

A method for determining carbendazim residue in watermelon and soil was developed by HPLC using external standard method for quantitation. Samples were extracted with the mixed solution of methanol and dilute hydrochloric acid. The extracts were cleaned up by liquid-liquid separation. The relation of carbendazim peak area and sample injection concentration exhibited linearity in a range from 0.005 to 2.0 mg/L.The detection limit of carbendazim was 0.01 mg/kg for watermelon, and 0.02 mg/kg for soil. Test results of spiked recoveries of three mass levels indicated that average spiked recovery rates of carbendazimin ranged from 83.46% to 104.84% for watermelon, and the RSD was from 2.28% to 6.03%; average spiked recovery rates of carbendazimin ranged from 84.70% to 102.36% for soil, and the RSD was from 2.01% to 5.45%.