Simultaneously Analysis of Residue of Carbendazim and Thiram in Fruits by High Performance Liquid Chromatography

A specific high performance liquid chromatographic(HPLC) method was developed for simultaneous determination of two fungicides including,carbendazim and thiram,in fruit samples.Residue of two fungicides was extracted from fruits with dichloromethane,cleaned up by Oasis HLB solid-phase extraction(SPE),and separated on a C18 column(250 mm×4.6 mm i.d.,5 μm) using methanol-0.05 mol/L ammonium formate(60 ∶40,by volume) as mobile phase at a flow rate of 1.0 mL/min.The detection wavelength was set at 285 nm.The method showed good linearity over the concentration range of 0.01-10.0 mg/kg for carbendazim with correlation coefficient of 1.0,and 0.05-10.0 mg/kg for thiram with correlation coefficient of 0.999 9.The limit of detections for carbendazim and thiram were 0.005 mg/kg and 0.03 mg/kg,respectively.The average recoveries of two fungicides in spiked samples were between 80% and 109%.The developed method was successfully applied to detect the target compounds in real samples.