Determination of Carbendazim Residue in Paddy Water, Soil and Rice by HPLC

A method for determining carbendazim residue in water,soil,and rice was developed by HPLC using external standard method for quantitation.Samples were extracted with the mixed solution of methanol and dilute hydrochloric acid.The extracts cleaned up by liquid to liquid separation.The relation of carbendazim peak area and sample injective quality exhibited linearity in a range between 0.15 ng and 220 ng.The minimum detection mass ratio of carbendazim for paddy water was 0.01 mg/kg,for soils 0.01 mg/kg and for rice 0.02 mg/kg.Test results of spiked recoveries of 3 levels indicated that average spiked recoveries of carbendazim in paddy water ranged between 87.1% and 93.0%,in soils between 84.8% and 91.9%,and in rice between 83.9% and 89.6%.The RSD of carbendazim in paddy water ranged 3.3% and 3.8%,in soils between 1.4% and 4.1%,and in rice between 1.8% and 5.5%.