2010•Zhonghua zhongliu fangzhi zazhiRequires access
Rituximab sensitized navelbine-induced apoptosis in B-NHL cell lines
Hong Yu Zhang, Hong Tao Chen, Zhong Guan, Yan Huang, Xing Zhang, Tong Lin
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Abstract
OBJECTIVE:To investigate the sensitiziation of B-NHL cell lines to navelbine-induced apoptosis by rituximab and explore the mechanism.METHODS:Daudi,Ramos,Namalwa and Raji cells were treated with navelbine combined with or without rituximab.The inhibitory effects on cell proliferation were examined by XTT assay and the inhibition curves and the IC50 were generated.After treatment with rituximab for 24 hours,the expression of Bcl-2 in these cell lines was analyzed by Western blot.RESULTS:In Daudi,Namalwa and Raji cells,The values of IC50 of navelbine combined with rituximab were significantly lower than those of navelbine alone.Left shifting of the inhibition curves was observed when navelbine was combined with rituximab,indicating a synergistic effect between navelbine and rituximab.In addition,after treatment with rituximab for 24 hours,the expression of Bcl-2 protein was down-regulated in Raji and Namalwa cell lines.CONCLUSIONS:Rituximab can sensitize the navelbine-induced apoptosis in Daudi,Namalwa and Raji cell lines.Down-regulation of Bcl-2 expression might be involved in the mechanisms of the sensitization.