2013Journal of Inner Mongolia Agricultural UniversityRequires access

STUDY ON TRANSPLANTING TECHNOLOGY OF TISSUE CULTURE SEEDLING OF HASKAOP

Tian Rong-wei

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Abstract

Using the tissue culture with root aged for 65 days of HASKAOP as test materials in multi-factor orthogonal experiment,the purpose was to analyse the effect of different cultivation medium,methods of hardening plantlets and seasons on transplanting of HASKAOP,as well as the survival percent after the treatments of different hormones on transplanting of HASKAOP.The results showed that 59.0% survival percent was obtained in the cultivation medium of base soil with vermiculite: peat soil=1: 1 and in good growth condition,the average leaf blade increased 3.34 pieces.The survival percent in the third way of hardening plantlets was 65.0%,higher than other methods and 53.0% higher than the ck.The optimal time for transplanting of HASKAOP was spring and the survival percent was 62.0%.The survival percent of using100mg/L ABT before transplanting was 5.3% higher than the ck,but the transplanting efficiency of HASKAOP had barely improved with using other hormones.

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What this paper is about

Using the tissue culture with root aged for 65 days of HASKAOP as test materials in multi-factor orthogonal experiment,the purpose was to analyse the effect of different cultivation medium,methods of hardening plantlets and seasons on transplanting of HASKAOP,as well as the survival percent after the treatments of different hormones on transplanting of HASKAOP.The results showed that 59.0% survival percent was obtained in the cultivation medium of base soil with vermiculite: peat soil=1: 1 and in good growth condition,the average leaf blade increased 3.34 pieces.The survival percent in the third way of hardening plantlets was 65.0%,higher than other methods and 53.0% higher than the ck.The optimal time for transplanting of HASKAOP was spring and the survival percent was 62.0%.The survival percent of using100mg/L ABT before transplanting was 5.3% higher than the ck,but the transplanting efficiency of HASKAOP had barely improved with using other hormones.

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Available abstract

Using the tissue culture with root aged for 65 days of HASKAOP as test materials in multi-factor orthogonal experiment,the purpose was to analyse the effect of different cultivation medium,methods of hardening plantlets and seasons on transplanting of HASKAOP,as well as the survival percent after the treatments of different hormones on transplanting of HASKAOP.The results showed that 59.0% survival percent was obtained in the cultivation medium of base soil with vermiculite: peat soil=1: 1 and in good growth condition,the average leaf blade increased 3.34 pieces.The survival percent in the third way of hardening plantlets was 65.0%,higher than other methods and 53.0% higher than the ck.The optimal time for transplanting of HASKAOP was spring and the survival percent was 62.0%.The survival percent of using100mg/L ABT before transplanting was 5.3% higher than the ck,but the transplanting efficiency of HASKAOP had barely improved with using other hormones.

Key concepts: Transplanting, Vermiculite, Seedling, Biology, Horticulture, Germination, Agronomy, Botany

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