Separation,Purification,and Structural Analysis of PSP-1-A of Polygonatum Sibiricmu Polysaccharides

Objective: To discuss the extraction,separation and purification conditions of polygonatum sibiricmu polysaccharides,determine the molecular weight of the single polysaccharide,analyze the composition of the monosaccharide,and further preliminarily analyze its primary structure. Methods: Degrease the propared rhizoma polygonati by petroleum ether,apply water solution and alcohol sedimentation,DEAE-cellulose and Sephadex G-200 chromatographic column separation and purification,identify the purity with high performance liquid chromatography( HPLC),determine the molecular weight,detect the composition of monosaccharide with GC-MS,analyze the structure with infrared spectroscopy and nuclear magnetic resonance. Results: One polysaccharide was got with the molecular weight of 1410,the Mw was 8640,and the Mw / Mn was 6. 12. The monosaccharide was mainly composed by galactose and small amount of glc,rha,xyl,Ara and Man,with the molar ratio of 93. 8∶1. 4∶1. 3∶1. 5 ∶1. 1 ∶0. 9.The analysis of infrared spectroscopy and nuclear magnetic resonance indicates that PSP-1-A was the linear polysaccharide composed by β-1,4-gal and β-T-gal. Conclusion: PSP-1-A is a kind of polygonatum sibiricmu polysaccharide composed by new monosaccharide and of uniform relative molecular weight.