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Molecular Weight Determination and Monosaccharide Composition Analysis of Bupleurum chinense DC. Polysaccharide

Gou Xiao

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Abstract

[Objective] The aim was to extract and isolate Bupleurum chinense DC. polysaccharide (BPP2), and study its molecular weight and monosaccharide composition. [Method] BPP2 was extracted from B. chinense with water, isolated and purified by DEAE ion-exchange cellulose. The molecular weight of BPP2 was measured using GPC method, and TLC, HPLC were adopted to study its monosaccharide composition. [Result] The molecular weight of BPP2 was 67 836, which was composed of Rha, Xyl, Ara, Gal and Gle with molar ratio of 2.19∶1.00∶3.21∶2.27∶2.44. [Conclusion] BPP2 is pure heterosaccharide which is composed of five kinds of monosaccharide, the molecular weight is 67 836.

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What this paper is about

[Objective] The aim was to extract and isolate Bupleurum chinense DC. polysaccharide (BPP2), and study its molecular weight and monosaccharide composition. [Method] BPP2 was extracted from B. chinense with water, isolated and purified by DEAE ion-exchange cellulose. The molecular weight of BPP2 was measured using GPC method, and TLC, HPLC were adopted to study its monosaccharide composition. [Result] The molecular weight of BPP2 was 67 836, which was composed of Rha, Xyl, Ara, Gal and Gle with molar ratio of 2.19∶1.00∶3.21∶2.27∶2.44. [Conclusion] BPP2 is pure heterosaccharide which is composed of five kinds of monosaccharide, the molecular weight is 67 836.

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Available abstract

[Objective] The aim was to extract and isolate Bupleurum chinense DC. polysaccharide (BPP2), and study its molecular weight and monosaccharide composition. [Method] BPP2 was extracted from B. chinense with water, isolated and purified by DEAE ion-exchange cellulose. The molecular weight of BPP2 was measured using GPC method, and TLC, HPLC were adopted to study its monosaccharide composition. [Result] The molecular weight of BPP2 was 67 836, which was composed of Rha, Xyl, Ara, Gal and Gle with molar ratio of 2.19∶1.00∶3.21∶2.27∶2.44. [Conclusion] BPP2 is pure heterosaccharide which is composed of five kinds of monosaccharide, the molecular weight is 67 836.

Key concepts: Monosaccharide, Polysaccharide, Chemistry, Composition (language), Bupleurum, Chromatography, Cellulose, Molecular mass

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