A Study on the Apoptosis of BGC-823 Gastric Cancer Cells Induced by Sulindac

Objective To investigate the effect of sulindac on apoptsis of the human gastric cancer cells line-BGC-832 and its antineoplastic mechanisma. Methods Sulindac were incubated with human gastric cancer cell line-BGCC-823 in various concentrations for different time. The positive rates of apoptosis were determined by flow cytometry and TUNEL assay,expressions of apoptosis related genes protein,including bcl-2 and survivin were analysed by immunohistochemical staining,expressions of COX-2 protein was used to detect immunohistochemical staining. Results The apex of apoptosis was determined by flow cytometry. The rate of apoptosis in gastric cancer cell line-BGC-823 was significantly higher than that in comparison group by flow cytometry and TUNEL assay(P0.05). Some morphologic features of apoptotic and the a pportosis body were examined by transmission electron microscopy. The expressions of bcl-2, survivin and COX-2 were decreased in Sulindac treated cells as compared with the untreated control cells. All the effects were in a time-and dose-dependent manner(P0.05). Conclusion Sulindac can induce apoptosis in gastric cancer cell line-BGC-823 by inhibit apoptosis related genes protein, including bcl-2 and survivin expression, downergulation of COX-2 expression.